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Image Search Results
Journal: Journal of Neuroinflammation
Article Title: Metformin inhibits intracranial aneurysm formation and progression by regulating vascular smooth muscle cell phenotype switching via the AMPK/ACC pathway
doi: 10.1186/s12974-020-01868-4
Figure Lengend Snippet: Metformin inhibits VSMC proliferation, migration, and apoptosis via AMPK activation. A Cell proliferation assay measured with CCK-8 at 0.5, 1, 2, and 4 h after incubated with 5, 50, and 500 μM metformin and compound C. B Evaluation of migratory capacity with the transwell assay. (a–e) Representative images of migrating cells stained with crystal violet are shown for the control (a), 500 μM metformin (b), 50 μM metformin (c), 5 μM metformin (d), and 10 μM compound C (e) groups. Scale bar = 50 μM. C (a–e) Representative images and quantitative analysis of apoptotic cells analyzed by Hoechst staining in the control (a), 500 μM metformin (b), 50 μM metformin (c), 5 μM metformin (d), and 10 μM compound C (e) groups. Scale bar = 50 μM. (f) Quantitative analysis. * P < 0.05 vs control group; # P < 0.05 vs 500 μM metformin group. OD, optical density
Article Snippet: Cell migration was assessed with
Techniques: Migration, Activation Assay, Proliferation Assay, CCK-8 Assay, Incubation, Transwell Assay, Staining
Journal: Journal of Neuroinflammation
Article Title: Metformin inhibits intracranial aneurysm formation and progression by regulating vascular smooth muscle cell phenotype switching via the AMPK/ACC pathway
doi: 10.1186/s12974-020-01868-4
Figure Lengend Snippet: VSMC proliferation, migration, and apoptosis are regulated via AMPK/ACC signaling. A Cell viability following treatment with ND-646 and ND-646 + metformin at indicated times. B Evaluation of VSMC migration with the transwell assay. Scale bar = 50 μM). (a–d) Representative images of control (a), (b) ND-646 (b), and ND-646 + 500 μM metformin (c) groups and quantitative analysis (d). C Apoptotic cells detected by Hoechst staining. Scale bar = 50 μM. (a–d) Representative images of control (a), (b) ND-646 (b), and ND-646 + 500 μM metformin (c) groups and quantitative analysis (d). * P < 0.05 vs control group. OD, optical density
Article Snippet: Cell migration was assessed with
Techniques: Migration, Transwell Assay, Staining